Development of Shoot Cultures from Leaf Explant of Portulaca quadrifida L.

  • Ashutosh PATHAK The Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002, Gujarat
  • Aruna JOSHI The Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002, Gujarat
  • Asha SHARMA The Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002, Gujarat
Keywords: carbon source, indirect organogenesis, leaf explant, Murashige and Skoog, shoot cultures

Abstract

Portulaca quadrifida (Portulacaceae) is an annual succulent herb having medicinal value and is consumed as a vegetable or salads in India. In the present study, leaf explants were inoculated on Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and combinations of N6-benzyladenine (6-BA) and kinetin (KIN) individually and in combination with 1-naphtalene acetic acid (NAA). Rapid regeneration was observed in medium fortified with combinations of 6-BA (8 µM) and NAA (1 µM) which formed 19.40 ± 0.64 shoots with 100% response. Variation in sucrose concentrations (4-6%) was tried but it failed to increase the shoot number. When the optimized medium was fortified with different carbon sources viz. dextrose, glucose and maltose, they could not evoked better response and sucrose proved to be more effective for regeneration. Rooting of in vitro shoots was achieved in ½MS + sucrose (1%) + indole-3-butyric acid (IBA, 2 µM).

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Published
2019-03-21
How to Cite
PATHAK, A., JOSHI, A., & SHARMA, A. (2019). Development of Shoot Cultures from Leaf Explant of Portulaca quadrifida L. Notulae Scientia Biologicae, 11(1), 45-50. https://doi.org/10.15835/nsb11110332
Section
Research articles