Assessment of Aglycones Isoflavone Profiling of Staple Indian Grain Flours and Soybean Sprout-Flour

Authors

  • Raman MANOHARLAL ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)
  • Saiprasad V.S. GANDRA ITC Limited, ITC Life Science and Technology Centre (LSTC), Peenya Industrial Area, 1st Phase, Bengaluru-560058, Karnataka (IN)

DOI:

https://doi.org/10.15835/nsb10410331

Keywords:

daidzein; flours; genistein; glycitein; HPLC; isoflavones

Abstract

In the present study, investigation of fourteen traditional and most commonly used Indian staple grain flour types (viz. wheat, white rice, processed little millet, maize, all-purpose/refined wheat flour, chickpea flour, toasted gram flour, little millet, quinoa, soybean, white millet, pearl millet, semolina/cream of wheat and finger millet) was undertaken for the assessment of 3 major bioactive aglycone forms of isoflavone (IF): daidzein (DI), glycitein (GY) and genistein (GN), with a special interest on the effect of sprouting on total and individual IF components. The obtained results showed that the content and composition of total IF were negligible among all the investigated flours except for soybean, wherein detectable total (227 mg kg-1) and individual IF (45, 129 and 53 mg kg-1 for DI, GY and GN respectively) components were observed. From soybean mature seeds to sprouts formation with ~80% germination rate at a pilot-scale, a 31% increase in total IF (298 mg kg-1), characterised by an individual and respective increment of 30% (58 mg kg-1), 25% (161 mg kg-1) and 48% (78 mg kg-1) in corresponding DI, GY and GN components, was observed. The current results demonstrated that for the Indian scenario, contribution of aforementioned grains, other than soybean in daily dietary intake of IF is negligible and sprouting represents an effective way to enhance the endogenous IF content.

Metrics

Metrics Loading ...

References

Agrahar-Murugkar D, Jha K (2009). Effect of sprouting on nutritional and functional characteristics of soybean (Glycine max L). Journal of Food Science and Technology 46(3):240-243.

Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R, Kinae N (2000). Dietary intakes of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol concentration. The Journal of Nutrition 130(9):2243-2250.

Bau H, Villaume C, Nicolas J, Méjean L (1997). Effect of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean (Glycine max) seeds. Journal of the Science of Food and Agriculture 73(1):1-9.

Bau HM, Villaume C, Méjean L (2000). Effects of soybean (Glycine max) germination on biologically active components, nutritional values of seeds, and biological characteristics in rats. Food / Nahrung 44(1):2-6.

Bhagwat S, Haytowitz DB, Holden JM (2008). USDA database for the isoflavone content of selected foods release 2.0. Maryland: US Department of Agriculture pp 15.

Chen H, Seguin P, Jabaji S, Liu W (2011). Spatial distribution of isoflavones and isoflavone-related gene expression in high-and low-isoflavone soybean cultivars. Canadian Journal of Plant Science 91(4):697-705.

Chiarello MD, Le Guerroué J-l, Chagas CMSFranco OL, Bianchini E, Joao MJ (2006). Influence of heat treatment and grain germination on the isoflavone profile of soy milk. Journal of Food Biochemistry 30:234-247.

Cos P, De Bruyne T, Apers S, Berghe DV, Pieters L, Vlietinck AJ (2003). Phytoestrogens: recent developments. Planta Medica 69:589-599.

Crozier A, Jaganath IB, Clifford MN (2009). Dietary phenolics: chemistry, bioavailability and effects on health. Natural Product Reports 26:1001-1043.

de Kleijn MJ, van der Schouw, Yvonne T, Wilson PW, Adlercreutz H, Mazur W, Grobbee DE, Jacques PF (2001). Intake of dietary phytoestrogens is low in postmenopausal women in the United States: The Framingham study. The Journal of Nutrition 131(6):1826-1832.

Dikshit M, Ghadle M (2003). Effect of sprouting on nutrients, antinutrients and in vitro digestibility of the MACS-13 soybean variety. Plant Foods for Human Nutrition 58(3):1-11.

Duncan AM, Phipps WR, Kurzer MS (2003). Phyto-oestrogens: best practice and research. The Journal of Clinical Endocrinology & Metabolism 17(2):253-271.

Duyff RL (2012). Flour power: Learn about different kinds of flours. Retrievd 27 Febr 2018 from https://foodandnutrition.org/summer-2012/flour-power-learn-different-kinds-flours/

Franke AA, Custer LJ, Cerna CM, Narala KK (1994). Quantitation of phytoestrogens in legumes by HPLC. Journal of Agricultural and Food Chemistry 42(9):1905-1913.

Friedman M, Brandon DL (2001). Nutritional and health benefits of soy proteins. Journal of Agricultural and Food Chemistry 49(3):1069-1086.

Gandhi A (2008). Development of HACCP procedure for the production of full fat soy flour. International Food Research Journal 15(2):141-154.

Gao Y, Yao Y, Zhu Y, Ren G (2015). Isoflavone content and composition in chickpea (Cicer arietinum L.) sprouts germinated under different conditions. Journal of Agricultural and Food Chemistry 63(10):2701-2707.

Garci¬a-Lafuente A, Guillamón E, Villares A, Rostagno MA, Martínez JA (2009). Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflammation Research 58(9):537-552.

Guerroué JLL, Chagas CMS, Franco OL, Bianchini E, João MJ (2006). Influence of heat treatment and grain germination on the isoflavone profile of soy milk. Journal of Food Biochemistry 30(2):234-247.

Ho HM, Chen RY, Leung LK, Chan FL, Huang Y, Chen ZY (2002). Difference in flavonoid and isoflavone profile between soybean and soy leaf. Biomedicine & Pharmacotherapy 56(6):289-295.

Ho SC, Woo JL, Leung SS, Sham AL, Lam T, Janus E (2000). Intake of soy products is associated with better plasma lipid profiles in the Hong Kong Chinese population. The Journal of Nutrition 130(10):2590-2593.

Hwang CS, Kwak HS, Lim HJ, Lee SH, Kang YS, Choe TB, Hur HG, Han KO (2006). Isoflavone metabolites and their in vitro dual functions: they can act as an estrogenic agonist or antagonist depending on the estrogen concentration. The Journal of Steroid Biochemistry and Molecular Biology 101(4-5):246-253.

International Seed Testing Association (ISTA) (1999). International rules for seed testing. Seed Science and Technology 27 (Supplement):1-333.

Kalaiselvan V, Kalaivani M, Vijayakumar A, Sureshkumar K, Venkateskumar K (2010). Current knowledge and future direction of research on soy isoflavones as a therapeutic agents. Pharmacognosy Reviews 4(8):111-117.

Kaur M, Badhan RKS (2015). Phytoestrogens modulate breast cancer resistance protein expression and function at the blood-cerebrospinal fluid barrier. Journal of Pharmacy & Pharmaceutical Sciences 18(2):132-154.

Kulling SE, Lehmann L, Metzler M (2002). Oxidative metabolism and genotoxic potential of major isoflavone phytoestrogens. Journal of Chromatography B 777(1-2):211-218.

Kumar V, Rani A, Dixit AK, Bhatnagar D, Chauhan G (2009). Relative changes in tocopherols, isoflavones, total phenolic content, and antioxidative activity in soybean seeds at different reproductive stages. Journal of Agricultural and Food Chemistry 57(7): 2705-2710.

Kuo Y, Rozan P, Lambein F, Frias J, Vidal-Valverde C (2004). Effects of different germination conditions on the contents of free protein and non-protein amino acids of commercial legumes. Food Chemistry 86(4):537-545.

Liggins J, Bluck L, Runswick S, Atkinson C, Coward W, Bingham S (2000). Daidzein and genistein contents of vegetables. British Journal of Nutrition 84(5):717-725.

Liggins J, Mulligan A, Runswick S, Bingham S (2002). Daidzein and genistein content of cereals. European Journal of Clinical Nutrition 56(10):961.

Mazur W (1998). Phytoestrogen content in foods. Baillière's Clinical Endocrinology and Metabolism 12(4):729-742.

Mazur WM, Duke JA, Wähälä K, Rasku S, Adlercreutz H (1998). Isoflavonoids and lignans in legumes: Nutritional and health aspects in humans 1. The Journal of Nutritional Biochemistry 9(4):193-200.

Mira L, Tereza Fernandez M, Santos M, Rocha R, Helena Florêncio M, Jennings KR (2002). Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. Free Radical Research 36(11):1199-1208.

Müllner C, Sontag G (2000). HPLC with coulometric electrode array detection determination of daidzein and genistein in soy based infant food, soy milk and soy based supplements. European Food Research and Technology 211(4):301-304.

Nagata C, Takatsuka N, Kawakami N, Shimizu H (2001). Soy product intake and hot flashes in Japanese women: results from a community-based prospective study. American Journal of Epidemiology 153(8):790-793.

Strom SS, Yamamura Y, Duphorne CM, Spitz MR, Babaian RJ, Pillow PC, Hursting SD (2000). Phytoestrogen intake and prostate cancer: A case-control study using a new database. Nutrition and Cancer 36(2):243.

Terashima M, Kakuno Y, Kitano N, Matsuoka C, Murase M, Togo N, Watanabe R, Matsumura S (2012). Antioxidant activity of flavonoids evaluated with myoglobin method. Plant Cell Reports 31(2):291-298.

Vacek J, Klejdus B, Lojková L, Kubán V (2008). Current trends in isolation, separation, determination and identification of isoflavones: a review. Journal of Separation Science 31(11):2054-2067.

Yamamoto S, Sobue T, Sasaki S, Kobayashi M, Arai Y, Uehara M, Adlercreutz H, Watanabe S, Takahashi T, Iitoi Y (2001). Validity and reproducibility of a self-administered food-frequency questionnaire to assess isoflavone intake in a Japanese population in comparison with dietary records and blood and urine isoflavones. Journal of Nutrition 131:2741-2747.

Downloads

Published

2018-12-21

How to Cite

MANOHARLAL, R., & GANDRA, S. V. (2018). Assessment of Aglycones Isoflavone Profiling of Staple Indian Grain Flours and Soybean Sprout-Flour. Notulae Scientia Biologicae, 10(4), 485–492. https://doi.org/10.15835/nsb10410331

Issue

Section

Research articles
CITATION
DOI: 10.15835/nsb10410331