Purification, Characterization and Antioxidant Potential of a Novel Lectin from Pterocarpus soyauxii Taub Seeds
DOI:
https://doi.org/10.15835/nsb11110317Keywords:
antioxidant, hemagglutination, lectin, Pterocarpus soyauxii, seedAbstract
The study investigated the presence of lectin in the seeds of Pterocarpus soyauxii, purified the lectin and determined its physicochemical properties as well as its antioxidant potential. Purification of P. soyauxii seeds lectin (PSSL) was accomplished by ammonium sulphate precipitation and gel filtration on Sephadex G-100, hemagglutination assay was by serial dilution and DPPH radical scavenging, metal chelating and lipid peroxidation inhibition assays were employed to evaluate its antioxidant potential. PSSL agglutinated erythrocytes from human blood group ABO, rabbit and rat. Trypsinization enhanced agglutination which was inhibited by galactose, lactose and arabinose. The lectin activity was independent of metal ion, thermal stable up to 60 °C and at pH range of 6 to 8. Gel filtration estimated molecular weight was 65 kDa and subunit molecular weight was 35 kDa. PSSL showed significant antioxidant activity. The lectin was able to scavenge DPPH radical, chelate iron metal and inhibit lipid peroxidation with IC50 of 0.14 ± 1.33 mg/ml, 3.40 ± 0.37 mg/ml and 0.50 ± 0.08 mg/ml respectively. The results indicate that PSSL might be applied as natural antioxidant for the development of drug and food products for the benefit of human health.
Metrics
References
Almeida AC, Osterne VJS, Santiago MQ, Pinto-Junior VR, Silva-Filho JS ... Cavada BS (2016). Structural analysis of Centrolobium tomentosum seed lectin with inflammatory activity. Archives of Biochemistry and Biophysics 596:73-83.
Anitha N, Sathisha GJ (2017). Evaluation of antibacterial and antioxidant activities of partially purified lectin from tubers of Xanthosoma violaceum. European Journal of Biomedical and Pharmaceutical Sciences 4(11):420-425.
Bashir H, Khan T, Masood A, Hamid (2010). Isolation, purification and characterization of a lectin from a local Kashmiri variety of soybean (Glycine max). Asian Journal of Biochemistry 5(3):145-153.
Benevides RG, Ganne G, Simoes RC, Schubert V, Niemietz M, ... Imberty A (2012). A lectin from Platypodium elegans with unusual specificity and affinity for asymmetric complex N-glycans. Journal of Biological Chemistry 287(31):26352-26363.
Bera AK, Rana T, Bhattacharya D, Das S, Pan D, Das SK (2011). Sodium arsenite-induced alteration in hepatocyte function of rat with special emphasis on superoxide dismutase expression pathway and its prevention by mushroom lectin. Basic and Clinical Pharmacology &Toxicology 109(4):240-244.
Bouwman LH, Roep BO, Roos A (2006). Mannose-binding lectin: clinical implications for infection, transplantation and autoimmunity. Human Immunology 67(4-5):247-256.
Brand-Williams W, Cuvelier ME, Besert CLWT (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1):25-30.
Calvete JJ, Santos CF, Mann K, Grangerio TB, Nimtz M, ... Cavada BS (1998). Amino acid sequence, glycan structure and proteolytic processing of the lectin of Vatairea macrocarpa seeds. FEBS Letter 425(2):286-292.
Carrasco-Castilla J, Hernandez-Álvarez AJ, Jiménez-MartÃnez C, Jacinto-Hernández C, Alaiz M, ... Dávilla-Ortiz G (2012). Antioxidant and metal chelating activities of Phaseolus vulgaris L. var. Jamapa protein isolates, phaseolin and lectin hydrolysates. Food Chemistry 131(4):1157-1164.
Carvalho AS, da Silva MV, Gomes FS, Paiva PM, Malafaia CB, ... Correia MT (2015). Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds. International Journal of Biological Macromolecules 75:402-408.
Coffey DG, Uebersax MA, Hosfield GL, Bennick MR (1993). Thermal extrusion and alkali processing of dry beans (Phaseolus vulgaris). Journal of Food Processing and Preservatives 16(6):421-431.
Dan X, Liu W, Ng TB (2016). Development and applications of lectins as biological tools in biomedical research. Medicinal Research Reviews 36(2):221-247.
de Vasconcelos MA, Alves AC, Carneiro RF, Dias AHS, Martins FWV, .... Cavada BS (2015). Purification and primary structure of a novel mannose-specific lectin from Centrolobium microchaete Mart seeds. International Journal of Biological Macromolecules 81:600-607.
do Nascimento ASF, Gondim ACS, Cajazeiras JB, Correia JLA, Pires AF, .... Cavada BS (2012). Purification and partial characterization of a novel lectin from Dioclea lasiocarpa Mart seeds with vasodilator effects. Journal of Molecular Recognition 25(12):657-664.
Echemendia-Blanco D, Driessche EV, Ncube I, Read JS, Beeckmans S (2009). Stability, subunit interactions and carbohydrate-binding of the seed lectin from Pterocarpus angolensis. Protein and Peptide Letters 16(9):1120-1134.
e Lacerda RR, Moreira IC, de-Lacerda ACS, Cabral NL, Lucetti, DL, ... Santi-Gadelha T (2015). Lectin isolated from Brazilian seeds of velvet beans (Mucuna pruriens (L) DC.) presents analgesic, anti-inflamatory and antihemolytic action. Journal of Medicinal Plant Research 9(8):231-242.
e Lacerda RR, do Nascimento ES, de Lacerda JTJG, da Silva Pinto L, Rizzi C, ... De Almeida Gadelha TS (2017). Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. Var. cascavel) presents antioxidant, antitumor and gastroprotective activities. International Journal of Biological Macromolecules 95:1072-1081.
Engel M, Bachmann M, Schröder HC, Rinkevich B, Kljajic Z, ... Muller WEG (1992). A novel galactose-and arabinose-specific lectin from the sponge Pellina semitubulosa: isolation, characterization and immunological properties. Biochimie 74(6):527-537.
Galbraith W, Goldstein IJ (1970). Phytohemagglutinins: a new class of metalloprotein: Isolation, purification and some properties of the lectin from Phaseolus lunatus. FEBS Letter 9(4):197-201.
Gill LS (1992). Ethnomedical uses of plants in Nigeria. Uniben Press, University of Benin pp 200-201.
Gupta GS (2012). Animal lectins: forms, function and clinical application, Springer, Vienna pp 1108.
Hamid R, Masood A, Wani IH, Rafiq S (2013). Lectins: Proteins with diverse applications. Journal of Applied Pharmaceutical Science 3(4):S93-S103.
Hattori M, Yang XW, Miyashiro H, Namba T (1993). Inhibitory effects of monomeric and dimeric phenylpropanoids from mace on lipid peroxidation in vivo and in vitro. Phytotherapy Research 7(6):395-401.
Hendrickson OD, Zherdev AV (2018). Analytical application of lectin. Critical Reviews in Analytical Chemistry 48(4):279-292.
Huskens D, Schols D (2012). Algal lectins as potential HIV microbicide candidates. Marine Drugs 10(7):1476-1497.
Inbar J, Chet I (1997). Lectins and biocontrol. Critical Reviews in Biotechnology 17(1):1-20.
Joubert FJ, Sharon N, Merrifield EH (1986). Purification and properties of a lectin from Lonchocarpus capassa (apple leaf) seed. Phytochemistry 25(2):323-327.
Khurtsidze E, Kutchava T, Pomikalova N, Gaidamashvili M (2017). Galactose-binding lectin from mulberry (Morus alba L) seeds with growth hormone-like activity. Annals of Agrarian Science 15(1):26-30.
Kimura Y, Kubo M, Tani T, Arichi S, Okuda H (1981). Studies on Scutellariae radix. IV. Effects on lipid peroxidation in rat liver. Chemical and Pharmaceutical Bulletin 29(9):2610-2617.
Konozy EH, Mulay R, Faca V, Ward RJ, Greene LJ, ... Bhide SV (2002). Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin. Biochimie 84(10):1035-1043.
Konozy EH, Bernardes ES, Rosa C, Faca V, Greene LJ, Ward RJ (2003). Isolation, purification, and physicochemical characterization of a D-galactose-binding lectin from seeds of Erythrina speciosa. Archives of Biochemistry and Biophysics 410(2):222-229.
Kuku A, Eretan OB (2004). Purification and partial characterization of a lectin from the fresh leaves of Kalanchoe crenata (Andr.) Haw. Journal of Biochemistry and Molecular Biology 37(2):229-233.
Kuku A, Odekanyin OO, Okonji RE (2012). Physicochemical properties of a lactose specific lectin from the seeds of Erythrina senegalensis DC. Ife Journal of Science 14(1):143-153.
Kumar KK, Chandra KLP, Sumanthi J, Reddy GS, Shekar PC, Reddy B (2012). Biological roles of lectins: A review. Journal of Orofacial Sciences 4(1):20-25.
Lagarda-Diaz I, Guzman-Partida AM, Vazquez-Moreno L (2017). Legume lectins: protein with diverse applications. International Journal of Molecular Sciences 18(6):1242.
Lam SK, Ng TB (2011). Lectins: production and practical applications. Applied Microbiology and Biotechnology 89(1):45-55.
Lowry OH, Rosebrough NJ, Farr AL, Randall R (1951). Protein measurement using Folin phenol reagent. Journal of Biological Chemistry 193(1):265-275.
Mishra RP, Ganaie AA, Allaie AH (2016). Isolation and purification of a Galactose specific lectin from seeds of Bauhinia variegata and evaluation of its antimicrobial potential. International Journal of Pharmaceutical Science and Research 7(2):804-815.
Ng TB, Chan YS, Ng CCW, Wong JH (2015). Purification and characterization of a lectin from green split peas (Pisum sativum). Applied Biochemistry and Biotechnology 177(6):1374-1385.
Occena IV, Mojica EE, Merca FE (2007). Isolation and partial characterization of a lectin from the seeds of Artocarpus camansi Blanco. Asian Journal of Plant Sciences 6(5):757-764.
Odekanyin OO, Kuku A (2014). Characterization of galactose-specific lectin from the skin mucus of African catfish Clarias gariepinus. Scientific Research and Essays 9(20):869-879.
Olodude SO, Odekanyin OO, Fatokun AB (2017). Evaluation of antioxidant and carbohydrate hydrolysing enzymes inhibitory activities of Trilepisium madagascariense Ficalho seeds hemagglutinin. IOSR Journal of Pharmacy and Biological Sciences 12(6):29-41.
Osterne VJ, Santiago MQ, Pinto-Junior VR, Cajazeiras JB, Correia JL .... Cavada BS (2014). Purification, partial characterization and CNBr-Sepharose immobilization of a vasorelaxant glucose/mannose lectin from Canavalia virosa seeds. Applied Biochemistry and Biotechnology 172(7):3342-3353.
Osuagwu GGE, Akomas CB (2013). Antimicrobial activity of the leaves of three species of Nigerian Pterocarpus (Jacq.). International Journal of Medicinal and Aromatic Plants 3(2):178-183.
Pan WL, Ng TB (2015). A dimeric Phaseolus coccineus lectin with anti-oxidative, anti-proliferative and cytokine-inducing activities. International Journal of Biological Macromolecules 81:960-966.
Pattanapanyasat K, Noulsri E, Lerdwana S, Sukapirom K, Onlamoon N, Tassaneetrithep B (2010). The use of glutaraldehyde-fixed chicken red blood cells as counting beads for performing affordable single-platform CD4+ T-lymphocyte count in HIV-1-infected patients. Journal of Acquired Immune Deficiency Syndromes 53(1):47-54.
Pereira-Junior FN, Silva HC, Freitas BT, Rocha BAM, Nascimento KS, ... Cavada BS (2012). Purification and characterization of a mannose/N-acetyl-D-glucosamine-specific lectin from the seeds of Platymiscium floribundum Vogel. Journal of Molecular Recognition 25(8):443-449.
Petchiammal C, Hopper WAHEETA (2014). Antioxidant activity of proteins from fifteen varieties of legume seeds commonly consumed in India. International Journal of Pharmacy and Pharmaceutical Sciences 6(1-2):476-479.
Pompeu DG, Mattioli MA, Ribeiro RIMA, Goncalves DB, de Magalhaes JT ... Granjeiro PA (2015). Purification, partial characterization and antimicrobial activity of lectin from Chenopodium quinoa seeds. Food Science and Technology (Campinas) 35(4):696-703.
Sadananda TS, Govindappa M, Ramachandra YL (2014). In vitro antioxidant activity of lectin from different endophytic fungi of Viscum album L. British Journal of Pharmaceutical Research 4(5):626.
Saha RK, Acharya S, Jamiruddin M, Roy P, Islam MMS, ... Shovon, SSH (2014). Antimicrobial effects of a crude plant lectin isolated from the stem of Tinospora tomentosa. Journal of Phytopharmacology 3:44-51.
Saliu JA, Elekofehinti OO, Komolafe K, Oboh G (2012). Effects of some green leafy vegetables on the haematological parameters of diabetic rats. Journal of Natural Product and Plant Resources 2(4):482-485.
Sampaio AH, Rogers DJ, Barwell CJ (1998). A galactose-specific lectin from the red marine alga Ptilota filicina. Phytochemistry 48(5):765-769.
Santiago MQ, Leitão CC, Pereira-Junior FN, Pinto-Junior VR, Osterne JS, ... Cavada BS (2014). Purification, characterization and partial sequence of a pro-inflammatory lectin from seeds of Canavalia oxyphylla Standl. & LO Williams. Journal of Molecular Recognition 27(3):117-123.
Santos AFS, Argolo ACC, Coelho LCBB, Paiva PM (2005). Detection of water-soluble lectin and antioxidant component from Moringa oleifera seeds. Water Research 39(6):975-980.
Santos AFS, da Silva MDC, Napoleão TH, Paiva PMG, Correia MTS, Coelho LCBB (2014). Lectins: function, structure, biological properties and potential application. Current Topics in Peptide and Protein Research 15:41-62.
Shanmugavel S, Velayutham V, Kamalanathan T, Periasamy M, Munusamy A, Sundaram J (2016). Isolation and analysis of mannose/trehalose/maltose specific lectin from jack bean with antibruchid activity. International Journal of Biological Macromolecules 91:1-14.
Sharon N, Lis H (2004). History of Lectins: From hemagglutinins to biological recognition molecules. Glycobiology 14(1):53R-62R.
Silva HC, Nagano CS, Souza LA, Nascimento KS, IsÃdro R, ... Damasceno LEA (2012a). Purification and primary structure determination of a galactose-specific lectin from Vatairea guianensis Aublet seeds that exhibits vasorelaxant effect. Process Biochemistry 47(12):2347-2355.
Silva MC, Santana LA, Mentele R, Ferreira RS,de Miranda A, ... Oliva MLV (2012b). Purification, primary structure and potential functions of a novel lectin from Bauhinia forficata seeds. Process Biochemistry 47(7):1049-1059.
Silva HC, Bari AU, Rocha BAM, Nascimento KS, Ponte EL, Pires AF, ... Nagano CS (2013). Purification and primary structure of a mannose/glucose-binding lectin from Parkia biglobosa Jacq. Seeds with antinociceptive and anti-inflammatory properties. Journal of Molecular Recognition 26(10):470-478.
Singh RS, Tiwary AK, Kennedy JF (1999). Lectins: Sources, activities and applications. Critical Reviews in Biotechnology 19(2):145-178.
Singh N, Rajini PS (2004). Free radical scavenging activity of an aqueous extract of potato peel. Food Chemistry 85(4):611-616.
Singh AP, Saxena KD (2013). Biological Activity of Purified Momardica Charantia Lectin. Chemical Science Transactions 2:258-262.
Sun J, Yang QL, Bi J, Zhang CS, Yu LN, Zhu F (2011). Purification and identification of a natural lectin from the seed of peanut Arachis hypogea. The Open Materials Science Journal 5:78-82.
Tchamadeu MC, Dzeufiet PDD, Nana P, Nouga CK, Tsofack FN, ... Tack I (2011). Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papilionaceae) in rodents. Journal of Ethnopharmacology 133(2):329-335.
Teixeira-Sá DM, Reicher F, Braga RC, Beltramini LM, deAzavedo MR (2009). Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds. Phytochemistry 70(17-18):1965-1972.
Uzodimma D (2013). Medico-ethnobotanical inventory of Ogii, Okigwe Imo State, South Eastern Nigeria-I. Global Advanced Research Journal of Medicinal Plants (GARJMP) 2:030-044.
Wang HX, Liu WK, Ng TB, Ooi VEC, Chang ST (1996). The immunomodulatory and antitumour activities of lectins from the mushroom Tricholoma mongolicum. Immunopharmacology 31(2-3):205-211.
Wang H, Ng TB (2005). First report of an arabinose specific fungal lectin. Biochemical Biophysical Research Communication 337(2):621-625.
Weber K, Osborn M (1975). Protein and sodium dodecyl sulphate: molecular weight determination on polyacrylamide gels and related procedures. Chapter 3 In “The Protein”. Edited by Neurath H and Hill RL. Academic press, New York, Vol 1, pp 179-223.
Yau T, Dan X, Ng CC, Ng TB (2015). Lectins with potential for anticancer therapy. Molecules 20(3):3791-3810.
Downloads
Published
How to Cite
Issue
Section
License
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
License:
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.
.png)
