Nutrient Competition Mediated Antagonism of Microbes Against Rhizoctonia solani

Mahesh S. YANDIGERI, Manoj Kumar SOLANKI, Sudheer KUMAR, Rajesh Kumar SINGH, Alok K. SRIVASTAVA

Abstract


Plant growth-promoting (PGP) microorganisms are beneficial soil micro creatures which may facilitate plant growth by direct or indirect ways. Bacillus amyloliquefaciens MB101 (BA), Streptomyces atrovirens N23 (SA) and Hypocrea lixii NAIMCC-F-01760 (HL) were earlier reported to have the ability to manage the tomato root rot disease caused by Rhizoctonia solani (RS) at some extent. In the present study, effect of nutrient supplementation like potato dextrose broth (PDB) and tomato root extract (TRE) on antagonism of these three microbes was characterized under the soil microcosm in order to understand the role of nutrient in microbe-microbe interaction. A moderate influence on the population of all three antagonists was resulted by PDB and TRE with RS. However, TRE and PDB were causing a significant impact on cell wall degrading enzymes and antifungal activity in the presence of RS. Moreover, hyphal degradation of RS was proved by scanning electron micrographs in the absence of substrates. Nutrient competition enhanced the call wall degrading enzyme production. Therefore, the present study concluded the role of substrate in the mycoparasitism and also sustain the potential of the hereby methodology (soil microcosm) for screening of other soil-inhibiting organism in the future.


Keywords


antifungal activity; chitinase; glucanase; potato dextrose broth; tomato root extract

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References


Almeida FB dos R, Cerqueira FM, Silva R do N, et al., Lima AL (2007). Mycoparasitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production. Biotechnology Letters 29(8):118993.

Arora NK, Khare E, Oh JH, Kang SC, Maheshwari DK (2008). Diverse mechanisms adopted by fluorescent Pseudomonas PGC2 during the inhibition of Rhizoctonia solani and Phytophthora capsici. World Journal of Microbiology and Biotechnology 24(4):581-585.

Bais HP, Weir TL, Perry LG, Gilroy S, Vivanco MJ (2006). The role of root exudates in rhizosphere Interactions with plants and other organisms. Annual Reviews in Plant Biology 57:233-266.

Boukaew S, Chuenchit S, Petcharat V (2011). Evaluation of Streptomyces spp. for biological control of Sclerotium root and stem rot and Ralstonia wilt of chili pepper. BioControl 56:365-374.

Cao R, Liu X, Gao K, Mendgen K, Kang Z, Gao J, et al., Wang X (2009). Mycoparasitism of endophytic fungi isolated from reed on soilborne phytopathogenic fungi and production of cell wall-degrading enzymes in vitro. Current Microbiology 59(6):584-592.

Compant S, Clément C, Sessitsch A (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry 42(5):669-678.

Doornbos RF, van Loon LC, Bakker PAHM (2012). Impact of root exudates and plant defense signaling on bacterial communities in the rhizosphere. A review. Agronomy for Sustainable Development 32(1):227-243.

Finkel OM, Castrillo G, Herrera Paredes S, et al., Dangl KL (2017). Understanding and exploiting plant beneficial microbes. Current Opinion in Plant Biology 38:155-163.

Gao K, Liu X, Kang Z, Mendgen K (2005). Mycoparasitism of Rhizoctonia solani by endophytic chaetomium spirale ND35: Ultrastructure and cytochemistry of the interaction. Journal of Phytopathology 153:280-290.

Harman G, Howell C, Viterbo A (2004). Trichoderma species--opportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2(1):43-56.

Hartmann A, Schmid M, Tuinen D van, Berg G (2009). Plant-driven selection of microbes. Plant Soil 321:235-257.

Huang Y, Kuang Z, Wang W, Cao L (2016). Exploring potential bacterial and fungal biocontrol agents transmitted from seeds to sprouts of wheat. Biological Control 98:27-33.

Kundan R, Pant G (2015). Plant growth promoting Rhizobacteria: Mechanism and current prospective. Journal of Fertilizers and Pesticides 6(2):9.

Lethbridge G, Bull AT, Burns RG (1978). Assay and properties of 1,3-β-glucanase in soil. Soil Biolog and Biochemistry 10(5):389-391.

Luo D-Q, Wang F, Bian X-Y, Liu J-K (2005). Rufuslactone, a new antifungal sesquiterpene from the fruiting bodies of the basidiomycete Lactarius rufus. The Journal of Antibiotics 58(7):456-459.

Malviya N, Yandigeri MS, Yadav AK, Solanki MK, Arora DK (2014). Isolation and characterization of novel alkali-halophilic actinomycetes from the Chilika brackish water lake, India. Annals of Microbiology 64(4):1829-1838.

Morton DJ, Stroube WH (1955). Antagonistic and stimulating effects of soil microorganisms upon Sclerotium rolfsii. Phytopathology 45:417-420.

Patil HJ, Solanki MK (2016a). Microbial inoculant: Modern era of fertilizers and pesticides. Microbial Inoculants in Sustainable Agricultural Productivity 319-343.

Patil HJ, Solanki MK (2016b). Molecular prospecting: Advancement in diagnosis and control of Rhizoctonia solani diseases in plants. Springer International Publishing pp 165-185.

Shoresh M, Harman G, Mastouri F (2010). Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review of Phytopathology 48:21-43.

Solanki MK, Kumar S, Pandey AK, et al., Arora DK (2012a). Diversity and antagonistic potential of Bacillus spp. associated to the rhizosphere of tomato for the management of Rhizoctonia solani. Biocontrol Science and Technology 22(2):203-217.

Solanki MK, Malviya MK, Wang Z (2016). Actinomycetes bio-inoculants: A modern prospectus for plant disease management. In Plant Growth Promoting Actinobacteria 36-81.

Solanki MK, Robert AS, Singh RK, et al., Arora DK (2012b). Characterization of mycolytic enzymes of Bacillus strains and their bio-protection role against Rhizoctonia solani in tomato. Current Microbiology 65(3):330-336.

Solanki MK, Singh N, Singh RK, et al., Arora DK (2011). Plant defense activation and management of tomato root rot by a chitin-fortified Trichoderma/Hypocrea formulation. Phytoparasitica 39(5):471-481.

Solanki MK, Singh RK, Srivastava S, et al., Arora DK (2014a). Isolation and characterization of siderophore producing antagonistic rhizobacteria against Rhizoctonia solani. Journal of Basic Microbiology 54(6):585-597.

Solanki MK, Singh RK, Srivastava S, et al., Arora DK (2014b). Isolation and characterization of siderophore producing antagonistic rhizobacteria against Rhizoctonia solani. Journal of Basic Microbiology 54(6):585-597.

Trotta A, Varese GC, Gnavi E, Fusconi A, Sampo S, Berta G (1996). Interactions between the soilborne root pathogenPhytophthora nicotianae var.parasitica and the arbuscular mycorrhizal fungus Glomus mosseae in tomato plants. Plant Soil 185(2):199-209.

Wen C-M, Tseng C-S, Cheng C-Y, Li Y-K (2002). Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnology and Applied Biochemistry 35(3):213-219.

Yandigeri MS, Malviya N, Solanki MK, Shrivastav P, Sivakumar G (2015). Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani. World Journal of Microbiology and Biotechnology 31(8):1217:1225.

Yu G, Sinclair J, Hartman G, Bertagnolli B (2002). Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biology and Biochemistry 34:955-963.




DOI: http://dx.doi.org/10.15835/nsb10310312


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Under the aegis of Horticulture and Forestry Society from Transylvania

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