Influence of Extraction Scheme on the Antioxidant Potential of Caralluma tuberculata


  • Sobia NOREEN University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Ishtiaq HUSSAIN University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Muhammad Ilyas TARIQ University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Shahid IQBAL University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Fozia BATOOL University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Shazia Akram GHUMMAN University of Sargodha, Faculty of Pharmacy, Sargodha 40100 (PK)
  • Shazia NOUREEN University of Sargodha, Department of Chemistry, Sargodha 40100 (PK)
  • Tusneem KAUSAR University of Sargodha, Department of Food Science and Technology, Sargodha 40100 (PK)



DPPH scavenging potential; microwave assisted extraction; phenolic; total flavonoid contents; ultrasonic assisted extraction


Herbal industry of developing countries is facing several technical issues related to the extraction conditions in order to attain the maximum yield of a plant extract with maximum therapeutic attributes. Therefore the present study was aimed to investigate the best technique for respective herbal products. The effect of three different extraction techniques: ultrasonic assisted extraction, microwave assisted and orbital shaker assisted extraction, by using three solvents (80% methanol, ethanol, and ethyl acetate) on the antioxidant potential of Caralluma tuberculata stem (non-conventional vegetable) extracts were investigated. Folin-Ciocalteu method was applied on tested samples in order to find the concentration of total phenols. Therefore, the optimized extract of high yield and maximum total phenolic content was selected for further analysis like total flavonoid contents, FRAP, DPPH and ABTS scavenging potential. Quantification of phenolic acids in the extracts was also carried out by HPLC. Significant variation was observed in the yield of total phenols within the extracts, but better results were obtained in aqueous methanolic extract of ultrasonic assisted extraction, followed by microwave assisted and orbital shaker assisted extraction. Present findings supported the view that ultrasonic assisted extraction can be used for phytochemicals profiling, activity guided assays and the development of herbal products. Correlation coefficients of active principles indicated a significant relationship to antioxidant capacity P < 0.05.


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How to Cite

NOREEN, S., HUSSAIN, I., TARIQ, M. I., IQBAL, S., BATOOL, F., GHUMMAN, S. A., NOUREEN, S., & KAUSAR, T. (2018). Influence of Extraction Scheme on the Antioxidant Potential of Caralluma tuberculata. Notulae Scientia Biologicae, 10(3), 340–347.



Research articles
DOI: 10.15835/nsb10310290