Essential Oil Composition and Antimicrobial Activity of Achillea biebersteinii Afan. (Asteraceae) from Erzincan Region, Turkey

Emre SEVİNDİK, Sinem AYDIN, Elif EREN APAYDIN, Mustafa SÜRMEN

Abstract


In the present study, chemical composition determination and in vitro antimicrobial effects of essential oil of Achillea biebersteinii plant grown under Erzincan ecological conditions were evaluated. Extractions were carried out with Clevenger apparatus and essential oil composition was determined by Gas Chromatography-Mass Spectrometry (GC-MS). Microorganisms used for the antimicrobial studies were Salmonella enterica serovar typhimirium ATCC 14028, Staphylococcus aureus subsp. aureus ATCC 25923, Yersinia pseudotuberculosis ATCC 911, Bacillus cereus 702 ROMA, Enterobacter aerogenes CCM 2531, Bacillus subtilis IMG 22 and Proteus vulgaris FMC 1. As a result, a total of 29 components were detected in Achillea biebersteinii.  Among them, 1,8-cineole (20.36%), cyclohexanone (8.39%), 2-cyclohexen-1-one (5.38%) and spathulenol (4.19%)  were found as the major components.  For the in vitro antimicrobial activity determination of essential oil, disc diffusion method was used in our study. Furthermore, 12-14 mm zone diameters were detected in antimicrobial activity assay. The highest resistance zone was detected against B. subtilis with 14 mm diameter while the least resistance zone was detected against Y. pseudotuberculosis, E. aerogenes and P. vulgaris with 12 mm diameter. Consequently, it was concluded that the essential oil extracted from the A. biebersteinii grown under Erzincan ecological conditions had an inhibitory effects on the pathogenic microorganisms in used method.  


Keywords


Achillea biebersteinii; antimicrobial activity; Erzincan; essential oil

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References


Arabaci A (2012). Achillea L. In: Guner A, Aslan S, Ekim T, Vural M, Babac MT (Eds). Turkiye Bitkileri Listesi (Damarli Bitkiler) Nezahat Gokyigit Botanik Bahcesi ve Flora Arastirmalari Dernegi Yayini, Istanbul.

Aytac Z, Duman H, Ekici M (2016). Two new Achillea L. (Asteraceae) species from Turkey. Turkish Journal of Botany 40:373-379.

Bader A, Flamini G, Cioni PL, Morelli I (2003). Essential oil composition of Achillea santolina L. and Achillea biebersteinii Afan. collected in Jordan. Flavour and Fragrance Journal 18(1):36-38.

Bağcı E, Koçak A, Yüce E (2008). The composition of the essential oils of two Achillea L. (Achillea wilhelmsii C. Koch. ve Achillea schischkinii Sosn.) species. Science and Engineering Journal of Firat University 20(2):251-255.

Balinova A, Dyakov G (1974). Improved apparatus for microdistillation of rose flower. Agricultural Science 11:79-85.

Bariş Ö, Güllüce M, Şahin F, Özer H, Kiliç H, Özkan H, Özbek T (2006). Biological activities of the essential oil and methanol extract of Achillea biebersteinii Afan. (Asteraceae). Turkish Journal of Biology 30(2):65-73.

Başer KHC, Demirci B, Demirci F, Güleryüz G (2002). Composition and antimicrobial activity of the essential oil of Achillea multifida. Planta Medica 68:941-943.

Bayaz M (2014). Essential oils: antimicrobial, antioxidant and antimutagenic activities. Academic Food Journal 12(3):45-53.

Bezic N, Skocibucic M, Dunkic V, Radonic A (2003). Composition and antimicrobial activity of Achillea clavennae L. essential oil. Phytotherapy Research 17:1037-1040.

Biçer A, Özkan G, Ergen A (2003). Lavanta bitkisi çiçeklerinden süperkritik CO2 ile uçucu yağların ekstraksiyonuna basıncın etkisi. G.Ü. Fen Bilimleri Dergisi 16(4):717-723.

Candan F, Ünlü M, Tepe B, Daferera D, Polissiou M, Sökmen A, Akpulat HA (2003). Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). Journal of Ethnopharmacology 87(2-3):215-220.

Celik N, Akpulat HA (2008). Achillea sivasica (Asteraceae: sect. Babounya (DC.) O. Hoffm.), a new species from inner Anatolia, Turkey. Kew Bulletin 63:485-489.

Çelik A, Çiçek M, Semiz G, Karıncalı M (2004). Taxonomical and ecological investigations on some geophytes growing around Denizli Province (Turkey). Turkish Journal of Botany 28:205-211.

Dobre AA, Gagiu V, Niculita P (2011). Preliminary studies on the antimicrobial activity of essential oils against food borne bacteria and toxigenic fungi. The Annals of the University Dunarea de Jos of Galati. Fascicle VI – Food Technology 35(2):16-26.

Ertürk Ö (2003). Scorzonera mollis Bieb. (Compositae) Bitkisinin Antimikrobiyal Aktivitesi, Çevre Koruma Dergisi 47:27-31.

Evren M, Tekgüler B (2011). Uçucu yağların antimikrobiyel özellikleri. Elektronik Mikrobiyoloji Dergisi 9(3):28-40.

Ghani A, Azizi M, Hassanzadeh-Khayyat M, Pahlavanpour AA (2008). Essential oil composition of Achillea eriophora, A. nobilis, A. biebersteinii and A. wilhelmsii from Iran. Journal of Essential Oil Bearing Plants 11(5):460-467.

Grassmann J, Elstner EF (2003). Essential Oils/properties and uses. Encyclopaedia of Food Science, Food Technology and Nutrition (Elsevier Science Ltd.) pp 2177-2184.

Kılıç A (2008). Uçucu yağ elde etme yöntemleri. Bartın Orman Fakültesi Dergisi 10(13):37-45.

Kordali Ş, Çakır A, Akcin TA, Mete E, Akcin A, Aydın T, Kılıç H (2009). Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor and Achillea bieberstenii Afan. (Asteraceae). Industrial Crops and Products 29(2-3):562-570.

Kotan R, Çakır A, Dadaşoğlu F, Aydın T, Çakmakçı R, Özer H, Kordali S, Mete E, Dikbaş N (2010). Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. Journal of the Science of Food and Agriculture 90(1):145-160.

Mazandarani M, Mirdeilami SZ, Pessarakli M (2013). Essential oil composition and antibacterial activity of Achillea millefolium L. from different regions in north east of Iran. Journal of Medicinal Plants Research 7(16):1063-1069.

Motavalizadehkakhky A, Shafaghat A, Zamani HA, Akhlaghi H, Mohammadhosseini, M., Mehrzad J, Ebrahimi Z (2013). Compositions and the in vitro antimicrobial activities of the essential oils and extracts of two Achillea species from Iran. Journal of Medicinal Plants Research 7(19):1280-1292.

Murray PR, Baron EJ, Pfaller MA, Tenovar FC, Yolke RH (1995). Manual of Clinical Microbiology. ASM Press, Washington D.C.

Nemeth E, Bernath J (2008). Biological activities of yarrow species (Achillea spp.). Current Pharmaceutical Design 14(29):3151-3167.

Nylinder S, Anderberg AA (2015). Phylogeny of the Inuleae (Asteraceae) with special emphasis on the Inuleae-Plucheinae. Taxon 64(1):110-130.

Oussalah M, Caillet S, Saucier L, Lacroix M. (2006). Antimicrobial effects of selected plant essential oils on the growth of a Pseudomonas putida strain isolated from meat. Meat Science 73:236-244.

Öztürk M, Çetin Ö (2013). Inula tuzgoluensis (Asteraceae), a new species from Central Anatolia, Turkey. Turkish Journal of Botany 37:825-835.

Paksoy MY, Selvi S, Sevindik E, Uysal H (2016). Soil content in the Anacyclus L.(Asteraceae) genus growing in Turkey. Turkish Journal of Life Sciences 1(1):8-12.

Picman AK (1986). Biological activities of sesquiterpene lactones. Biochemical Systematics and Ecology 14(3):225-281.

Rustaiyan A, Komeilizadeh H, Shariatpanahi MS, Jassbi AR, Masoudi S (1998). Comparative study of the essential oils of three Achillea species from Iran. Journal of Essential Oil Research 10(2):207-209.

Saric CL, Cabarkapa SI, Beljkas MB, Misan CA, Sakac BM, Plavsic VD (2009). Antimicrobial activity of plant extracts from Serbia. Food Processing Quality and Safety 1(2):1-5.

Shing B, Sahu PM, Sharma MK (2002). Anti-inflammatory and antimicrobial activities of Triterpenoids from Strobilanthes callosus Ness. Phytomedicine 9:355-359.

Si XT, Zhang ML, Shi QW, Kiyota H (2006). Chemical constituents of the plants in the genus Achillea. Chemistry and Biodiversity 3:1163-1179.

Sökmen A, Sökmen M, Daferera D, Polissiou M, Candan F, Ünlü M. Akpulat HA (2004). The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteinii Afan. (Asteraceae). Phytotheraphy Research 18:451-456.

Srivastava P, Logesh AR, Upreti DK, Dhole TN, Srivastava A (2013). In vitro evaluation of some Indian lichens against human pathogenic bacteria. Mycosphere 4(4):734-743.

Tabanca N, Demirci B, Aytaç Z, Baser KHC (2016). Chemical composition of Achillea schischkinii Sosn., an endemic species from Turkey. Natural Volatiles and Essential Oils 3:24-28.

Tabanca N, Demirci B, Gurbuez I, Demirci F, Becnel JJ, Wedge DE, Baser KH (2011). Essential oil composition of five collections of Achillea biebersteinii from central Turkey and their antifungal and insecticidal activity. Natural Product Communications 6(5):701-706.

Teneva D, Denkova Z, Goranov B, Denkova R, Kostov G, Atanasova T, Merdzhanov P (2016). Chemical composition and antimicrobial activity of essential oils from black pepper, cumin, coriander and cardamom against some pathogenic microorganisms. Acta Universitatis Cibiniensis Series E. Food Technology 20(2):39-52.

Toncer O, Basbag S, Karaman S, Diraz E, Basbag M (2010). Chemical composition of the essential oils of some Achillea species growing wild in Turkey. International Journal of Agriculture and Biology 12(4):527-530.

Vasinauskiene M, Radusiene J, Zitikaite I, Surviliene E (2006). Antibacterial activities of essential oils from aromatic and medicinal plants against growth of phytopathogenic bacteria. Agronomy Research 4:437-440.

Wallace RJ (2004). Antimicrobial properties of plant secondary metabolites. Proceeding of the Nutrition Society 63:621-629.

Yildirim B, Ekici K, Rezaeieh KAP (2015). Volatile oil components and antibacterial activity of Achillea biebersteinii Afan. from lake Van Basin, Turkey. Asian Journal of Chemistry 27(12):4686-4688.




DOI: http://dx.doi.org/10.15835/nsb10310222


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